Q: What is the primary function of the BD Rhapsody system?

A: The BD Rhapsody system is designed to perform single-cell RNA sequencing. It isolates individual cells using microfluidic chips, attaches unique barcodes to each cell's RNA, and prepares sequencing libraries to measure gene expression profiles for thousands of cells concurrently.

Q: How does the BD Rhapsody system's microfluidic chip technology work?

A: The system uses microfluidic chips to physically separate and encapsulate single cells into individual reaction chambers or droplets. Within these confined spaces, the cells' RNA is captured, reverse-transcribed, and labeled with a cell-specific barcode, ensuring that sequencing reads can be computationally assigned back to their cell of origin.

Q: How does BD Rhapsody compare to other single-cell RNA-seq platforms like 10x Genomics Chromium?

A: BD Rhapsody is distinguished by its use of microfluidic chips for cell encapsulation, while the 10x Genomics Chromium platform uses a droplet-based microfluidic system and Drop-seq employs hydrogel beads embedded with barcoded oligos. Each platform represents a different engineering approach to achieving the same goal of high-throughput single-cell transcriptome profiling.

Q: What biological research areas can be investigated using the BD Rhapsody system?

A: As a tool for single-cell RNA-seq, data generated with BD Rhapsody can be applied to study cellular diversity in developmental biology, characterize immune cell subsets in immunology, analyze tumor heterogeneity in cancer research, map neuronal types in neuroscience, and examine microbial communities in microbiology.

Q: Does using BD Rhapsody eliminate the common limitations of single-cell RNA-seq?

A: No. While BD Rhapsody provides a specific platform for executing scRNA-seq, experiments using this system are still subject to the general technical considerations of the technique. These include potential coverage bias toward highly expressed genes, technical noise from library preparation, high sequencing costs, and the need for sophisticated bioinformatics analysis.

BD Rhapsody

single-cell analysis system

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BD Rhapsody

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BD Rhapsody's subclass of is recorded as single-cell RNA-seq^[1].

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[1] ↑ BD Rhapsody — subclass of (P279): single-cell RNA-seq. wikidata.org.

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BD Rhapsody

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