# Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

> Research article (Nature Biotechnology, 2016) · cited 4,932× · AI/ML

**Wikidata**: [openalex:W2252568502](https://www.wikidata.org/wiki/openalex:W2252568502)  
**Source**: https://4ort.xyz/entity/optimized-sgrna-design-to-maximize-activity-and-minimize-off-target-effects-of-crispr-cas9