# Nellie: automated organelle segmentation, tracking and hierarchical feature extraction in 2D/3D live-cell microscopy

> Research article (Nature Methods, 2025) · cited 30× · AI/ML

**Wikidata**: [openalex:W4408062457](https://www.wikidata.org/wiki/openalex:W4408062457)  
**Source**: https://4ort.xyz/entity/nellie-automated-organelle-segmentation-tracking-and-hierarchical-feature-extraction-in-2d-3d-live-cell-microscopy